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Alpha Innotech alphaeasefc software
Alphaeasefc Software, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alphaeasefc+software/alphaeasefc+software/10__22175_slash_mmb__15701-93-6-8
Average 90 stars, based on 1 article reviews
alphaeasefc software - by Bioz Stars, 2026-09
90/100 stars

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Software:

Article Title: Heme oxygenase-1 emerges as a critical regulator linking autophagy dysregulation, immune infiltration, and NOD-Like receptor signaling in PCOS pathogenesis.
Article Snippet: Polycystic ovary syndrome (PCOS) is a prevalent endocrine-metabolic disorder affecting approximately 6–10% of women of reproductive age [1].. It is primarily characterized by hyperandrogenism, chronic anovulation, and polycystic ovarian morphology, all of which contribute to female infertility due to anovulation and hyperandrogenism [2].. Additionally, most PCOS patients develop peripheral insulin resistance (IR), increasing the risk of type 2 diabetes (DM2).

Article Title: Title Pending 15701
Article Snippet: Troponin-T immunoreactivity was detected by chemiluminescence (ECL Prime Western Blotting Detection Reagent Kit, GE Healthcare, Chicago, IL) using the FluorChem FC2 imaging system (Protein Simple). .. Densitometry measurements were performed using the AlphaEaseFC software (Alpha Innotech Corporation) with 1D Multi using 50% of the total lane width and a horizontal base with peak detection being adjusted manually. ..

Article Title: Human Endonuclease G Preferentially Cleaves Oxidatively Damaged DNA.
Article Snippet: .. The signal intensity of undigested plasmid DNA bands in the agarose gel was quantified using AlphaEaseFC software (version 4.0.0, Alpha Innotech) to calculate the percentage of degraded plasmid DNA. ..

Article Title: Therapeutic small molecules for treatment of pulmonary hypertension
Article Snippet: The immunoreactive bands were visualized with the Chemidoc XRS+system (Bio-Rad) using SuperSignal West Femto chemiluminescent substrates (Thermo Fisher Scientific). .. The images were later quantified using AlphaEaseFC software (Alpha Innotech). .. Plasmids: To construct a lentiviral plasmid carrying a GSTP1 transgene, oligonucleotides were synthesized by Integrated DNA Technologies.

Article Title: TLR4/NF-κB signaling-mediated neuroinflammation is associated with gut microbiota dysbiosis in a mouse model of Parkinson’s disease
Article Snippet: .. Protein bands were visualized using an automated chemiluminescence imaging system (Tanon5200, China), and the optical density of each band was analyzed semi-quantitatively using AlphaEaseFC software (Alpha Innotech, USA). ..

Article Title: HlncRNA-1 integrates the hepatic circadian clock with lipid metabolism by targeting Hdlbp.
Article Snippet: .. Quantification of Western blot was done using AlphaEaseFC software (AlphaInnotech, San Leando, CA, USA), and the grayscale values of target proteins were normalized to that of the β-Actin on the same membrane. .. All primary antibodies— β-Actin (Cat. No. HRP-66009, 1:10000 dilution), Bmal1 (Cat. No. 14268-1-AP, 1:2000 dilution), Per1 (Cat. No. 13463-1-AP, 1:1000 dilution), Per2 (Cat. No. 67513-1-Ig, 1:2000 dilution), Clock (Cat. No. 18094-1-AP, 1:1000 dilution), Nr1d1 (Cat. No. 14506-1-AP, 1:2000 dilution), Cry1 (Cat. No. 13474-1-AP, 1:2000 dilution), RORA (Cat. No. 82930-1-RR, 1:2000 dilution), RORC (Cat. No. 29910-1-AP, 1:2000 dilution) and β-Tubulin (Cat. No. 10094-1-AP, 1:5000 dilution) —were purchased from Proteintech (Wuhan, Hubei, China) Serological analysis Blood samples were collected in non-heparinized tubes and centrifuged at 1000×g for 10 minutes at 4 °C.

Article Title: Human Endonuclease G Preferentially Cleaves Oxidatively Damaged DNA
Article Snippet: .. The signal intensity of undigested plasmid DNA bands in the agarose gel was quantified using AlphaEaseFC software (version 4.0.0, Alpha Innotech) to calculate the percentage of degraded plasmid DNA. ..

Article Title: C-terminal HSP90 inhibitor NCT-58 impairs the cancer stem-like phenotype and enhances chemotherapy efficacy in TNBC
Article Snippet: .. Protein bands were detected using an Enhanced Chemiluminescence Kit (Thermo Fisher Scientific, Inc.), quantified using AlphaEaseFC software (Alpha Innotech Corporation), and normalized to GAPDH as the internal loading control. .. Cells cultured on 8-well chamber slides (BD Biosciences) were fixed with 4% paraformaldehyde at 4°C for 2 h, rinsed with PBS, and permeabilized with 0.2% Triton X-100 for 10 min.

Plasmid Preparation:

Article Title: Human Endonuclease G Preferentially Cleaves Oxidatively Damaged DNA.
Article Snippet: .. The signal intensity of undigested plasmid DNA bands in the agarose gel was quantified using AlphaEaseFC software (version 4.0.0, Alpha Innotech) to calculate the percentage of degraded plasmid DNA. ..

Article Title: Human Endonuclease G Preferentially Cleaves Oxidatively Damaged DNA
Article Snippet: .. The signal intensity of undigested plasmid DNA bands in the agarose gel was quantified using AlphaEaseFC software (version 4.0.0, Alpha Innotech) to calculate the percentage of degraded plasmid DNA. ..

Agarose Gel Electrophoresis:

Article Title: Human Endonuclease G Preferentially Cleaves Oxidatively Damaged DNA.
Article Snippet: .. The signal intensity of undigested plasmid DNA bands in the agarose gel was quantified using AlphaEaseFC software (version 4.0.0, Alpha Innotech) to calculate the percentage of degraded plasmid DNA. ..

Article Title: Human Endonuclease G Preferentially Cleaves Oxidatively Damaged DNA
Article Snippet: .. The signal intensity of undigested plasmid DNA bands in the agarose gel was quantified using AlphaEaseFC software (version 4.0.0, Alpha Innotech) to calculate the percentage of degraded plasmid DNA. ..

Imaging:

Article Title: TLR4/NF-κB signaling-mediated neuroinflammation is associated with gut microbiota dysbiosis in a mouse model of Parkinson’s disease
Article Snippet: .. Protein bands were visualized using an automated chemiluminescence imaging system (Tanon5200, China), and the optical density of each band was analyzed semi-quantitatively using AlphaEaseFC software (Alpha Innotech, USA). ..

Western Blot:

Article Title: HlncRNA-1 integrates the hepatic circadian clock with lipid metabolism by targeting Hdlbp.
Article Snippet: .. Quantification of Western blot was done using AlphaEaseFC software (AlphaInnotech, San Leando, CA, USA), and the grayscale values of target proteins were normalized to that of the β-Actin on the same membrane. .. All primary antibodies— β-Actin (Cat. No. HRP-66009, 1:10000 dilution), Bmal1 (Cat. No. 14268-1-AP, 1:2000 dilution), Per1 (Cat. No. 13463-1-AP, 1:1000 dilution), Per2 (Cat. No. 67513-1-Ig, 1:2000 dilution), Clock (Cat. No. 18094-1-AP, 1:1000 dilution), Nr1d1 (Cat. No. 14506-1-AP, 1:2000 dilution), Cry1 (Cat. No. 13474-1-AP, 1:2000 dilution), RORA (Cat. No. 82930-1-RR, 1:2000 dilution), RORC (Cat. No. 29910-1-AP, 1:2000 dilution) and β-Tubulin (Cat. No. 10094-1-AP, 1:5000 dilution) —were purchased from Proteintech (Wuhan, Hubei, China) Serological analysis Blood samples were collected in non-heparinized tubes and centrifuged at 1000×g for 10 minutes at 4 °C.

Membrane:

Article Title: HlncRNA-1 integrates the hepatic circadian clock with lipid metabolism by targeting Hdlbp.
Article Snippet: .. Quantification of Western blot was done using AlphaEaseFC software (AlphaInnotech, San Leando, CA, USA), and the grayscale values of target proteins were normalized to that of the β-Actin on the same membrane. .. All primary antibodies— β-Actin (Cat. No. HRP-66009, 1:10000 dilution), Bmal1 (Cat. No. 14268-1-AP, 1:2000 dilution), Per1 (Cat. No. 13463-1-AP, 1:1000 dilution), Per2 (Cat. No. 67513-1-Ig, 1:2000 dilution), Clock (Cat. No. 18094-1-AP, 1:1000 dilution), Nr1d1 (Cat. No. 14506-1-AP, 1:2000 dilution), Cry1 (Cat. No. 13474-1-AP, 1:2000 dilution), RORA (Cat. No. 82930-1-RR, 1:2000 dilution), RORC (Cat. No. 29910-1-AP, 1:2000 dilution) and β-Tubulin (Cat. No. 10094-1-AP, 1:5000 dilution) —were purchased from Proteintech (Wuhan, Hubei, China) Serological analysis Blood samples were collected in non-heparinized tubes and centrifuged at 1000×g for 10 minutes at 4 °C.

Control:

Article Title: C-terminal HSP90 inhibitor NCT-58 impairs the cancer stem-like phenotype and enhances chemotherapy efficacy in TNBC
Article Snippet: .. Protein bands were detected using an Enhanced Chemiluminescence Kit (Thermo Fisher Scientific, Inc.), quantified using AlphaEaseFC software (Alpha Innotech Corporation), and normalized to GAPDH as the internal loading control. .. Cells cultured on 8-well chamber slides (BD Biosciences) were fixed with 4% paraformaldehyde at 4°C for 2 h, rinsed with PBS, and permeabilized with 0.2% Triton X-100 for 10 min.



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